Published on Mon Jun 21 2021

Large-scale neural recordings with single-cell resolution in human cortex using high-density Neuropixels probes

Paulk, A. C., Kfir, Y., Khanna, A., Mustroph, M., Trautmann, E. M., Soper, D. J., Stavisky, S. D., Welkenhuysen, M., Dutta, B., Shenoy, K. V., Hochberg, L. R., Richardson, M., Williams, Z. M., Cash, S. S.

Recent advances in multi-electrode array technology have made it possible to monitor large neuronal ensembles at high resolution. Current approaches either restrict recordings to only a few neurons per penetrating electrode.

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Abstract

Recent advances in multi-electrode array technology have made it possible to monitor large neuronal ensembles at high resolution. In humans, however, current approaches either restrict recordings to only a few neurons per penetrating electrode or combine the signals of thousands of neurons in local field potential (LFP) recordings. Here, we describe a set of techniques which enable simultaneous recording from over 200 well-isolated cortical single units in human participants during intraoperative neurosurgical procedures using Neuropixels silicon probes. We characterized a diversity of extracellular waveforms with eight separable single unit classes, with differing firing rates, positions along the length of the linear electrode array, spatial spread of the waveform, and modulation by LFP events such as inter-ictal discharges and burst suppression. While some additional challenges remain in creating a turn-key system capable of recording, Neuropixels technology could pave the way to studying human-specific cognitive processes and their dysfunction at unprecedented spatiotemporal resolution.