Published on Fri Jul 30 2021

Collagen (I) homotrimer potentiates the osteogenesis imperfecta (oim) mutant allele and reduces survival in male mice

Lee, K. J., Rambault, L., Bou-Gharios, G., Clegg, P. D., Akhtar, R., Czanner, G., van 't Hof, R. J., Canty-Laird, E. G.

Osteogenesis imperfecta (oim) mouse model has a brittle bone phenotype. mice lacking the 2(I) chain do not have impaired bone biomechanical or structural properties, unlike oim homozygous mice. Mendelian inheritance was affected in male mice of both lines.

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Abstract

Type I collagen is the major structural component of bone where it exists as an (1)2(2)1 heterotrimer in all vertebrates. The osteogenesis imperfecta (oim) mouse model comprising solely homotrimeric (1)3 collagen-1, due to a dysfunctional 2 chain, has a brittle bone phenotype implying that the heterotrimeric form is required for physiological bone function. However, humans with null alleles preventing synthesis of the 2 chain have connective tissue and cardiovascular abnormalities (cardiac valvular Ehlers Danlos Syndrome), without evident bone fragility. Col1a2 null and oim mouse lines were used in this study and bones analysed by microCT and 3-point bending. RNA was also extracted from heterozygote tissues and allelic discrimination analyses performed using qRT-PCR. Here we show that mice lacking the 2(I) chain do not have impaired bone biomechanical or structural properties, unlike oim homozygous mice. However Mendelian inheritance was affected in male mice of both lines and male mice null for the 2 chain exhibited age-related loss of condition. The brittle bone phenotype of oim homozygotes could result from detrimental effects of the oim mutant allele, however, the phenotype of oim heterozygotes is known to be less severe. We used allelic discrimination to show that the oim mutant allele is not downregulated in heterozygotes. We then tested whether gene dosage was responsible for the less severe phenotype of oim heterozygotes by generating compound heterozygotes. Data showed that compound heterozygotes had impaired bone structural properties as compared to oim heterozygotes, albeit to a lesser extent than oim homozygotes. Hence, we concluded that the presence of heterotrimeric collagen-1 in oim heterozygotes alleviates the effect of the oim mutant allele but a genetic interaction between homotrimeric collagen-1 and the oim mutant allele leads to bone fragility.