Published on Thu Jun 24 2021

In planta genome editing with CRISPR/Cas9 ribonucleoproteins

Kumagai, Y., Liu, Y., Hamada, H., Luo, W., Zhu, J., Kuroki, M., Nagira, Y., Taoka, N., Kato, E., Imai, R.

In planta genome editing represents an attractive approach to engineering crops/varieties that are recalcitrant to culture-based transformation methods.

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Abstract

In planta genome editing represents an attractive approach to engineering crops/varieties that are recalcitrant to culture-based transformation methods. Here, we report the direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem using in planta particle bombardment and introduction of a semidwarf1 (sd1)-orthologous mutation into wheat. The triple knockout tasd1 mutant of an elite wheat variety reduced culm length by 10% without a reduction in yield.