Published on Fri Jul 30 2021

A small-molecule fluorescent probe for live-cell imaging of endocytosis

Seino, R., Iwashita, H., Takahashi, M., Ezoe, T., Ishiyama, M., Ueno, Y.

Study reports live-cell imaging of endocytosis using the newly-developed fluorescent probe ECGreen. ECGreen is not membrane permeable and its fluorescence signal increases in acidic conditions. It is a valuable new probe for endocytic imaging.

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Abstract

Endocytosis involves plasma membrane-derived vesicles for the recycling of intra- and extracellular components. Increasing evidence suggests that endocytosis is related to maintaining intracellular homeostasis and defense against disease. Consequently, investigation of the endocytic pathway attracts considerable scientific interest. This study reports live-cell imaging of endocytosis using the newly-developed fluorescent probe ECGreen. We demonstrate that ECGreen is not membrane permeable and its fluorescence signal increases in acidic conditions. Because of these characteristics, ECGreen remains on the plasma membrane, and then shows increased fluorescence when it is internalized into the acidic vesicles formed in the endocytic process. ECGreen allows direct observation of the internalized vesicle; it is a valuable new probe for endocytic imaging.