Published on Thu Sep 10 2020

Chromatin accessibility landscapes activated by cell surface and intracellular immune receptors

Ding, P., Sakai, T., Shrestha, R. K., Perez, N. M., Guo, W., Ngou, B. P. M., He, S., Liu, C., Feng, X., Zhang, R., Vandepoele, K., MacLean, D., Jones, J. D.

Activation of cell Surface and Intracellular Receptor-Mediated Immunity (SRMI and IRMI) results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which SRMI andIRMI lead to transcriptional changes are not clear.

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Abstract

Activation of cell Surface and Intracellular Receptor-Mediated Immunity (SRMI and IRMI) results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which SRMI and IRMI lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility; both SRMI and IRMI increase chromatin accessibility at induced defense genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by SRMI and IRMI, and by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanism between the two immune systems.